High Sensitivity Human IFN-γ (Interferon Gamma) ELISA Kit
中文名称：高敏型人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.98 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IFN-γ浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.98 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
IF: 2.082Journal： Journal of Zhejiang University-SCIENCE B (2020)Reactivity：HumanSample Type：Plasma -
Effects of grape juice consumption on oxidative stress and inflammation in male volleyball players: A randomized, double-blind, placebo-controlled clinical trial
IF: 2.063Journal： COMPLEMENTARY THERAPIES IN MEDICINE (2020)PMID：33183673Reactivity：HumanSample Type：serum -
Increased expression of IFN?γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：33335575Reactivity：HumanSample Type：Tissue homogenate -
Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells
IF: 1.287Journal： JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2020)PMID：32663414Reactivity：HumanSample Type：Cell culture supernatant -
MicroRNAs as Immune Regulators of Inflammation in Children with Epilepsy
Journal： International Journal of Molecular and Cellular Medicine (2020)Reactivity：HumanSample Type：plasma -
Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
IF: 2.431Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2019)PMID：31473895Reactivity：HumanSample Type：Cell lysate -
Efficacy of Intralesional Cryosurgery in the Treatment of Multiple Extragenital Cutaneous Warts: A Randomized Controlled Study
IF: 2.190 -
In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sj?gren’s syndrome
IF: 2.086 -
Intralesional vitamin D3 versus intralesional purified protein derivative in treatment of multiple warts: A comparative clinical and immunological study
IF: 1.740 -
MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis
IF: 3.967
实验操作视频
