High Sensitivity Human IFN-γ (Interferon Gamma) ELISA Kit
中文名称：高敏型人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.98 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IFN-γ浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.98 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Lymphocyte apoptosis and its association with the inflammatory markers and disease severity in juvenile-onset systemic lupus erythematosus patients
IF: 2.500Journal： Pediatric Rheumatology (2024) -
Serum mir-142-3p release in children with viral encephalitis and its relationship with nerve injury and inflammatory response
IF: 2.300Journal： JOURNAL OF NEUROVIROLOGY (2024)Reactivity：HumanSample Type：serum -
Pereskia bleo augments NK cell cytotoxicity against triple-negative breast cancer cells (MDA-MB-231)
IF: 2.300 -
Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3
IF: 2.000 -
circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity
IF: 1.500 -
Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment
IF: 10.900 -
Cryoablation inhibits the recurrence and progression of bladder cancer by enhancing tumour‐specific immunity
IF: 10.600Journal： Clinical and Translational Medicine (2023)PMID：37157934Reactivity：HumanSample Type：bladder tumour -
COPS6 promotes tumor progression and reduces CD8+ T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer
IF: 8.200Journal： ACTA PHARMACOLOGICA SINICA (2023)PMID：37095198PMC10462724Reactivity：MouseSample Type：MCF-7 cell-xenograft -
Sex Differences and Cytokine Profiles among Patients Hospitalized for COVID-19 and during Their Recovery: The Predominance of Adhesion Molecules in Females and Oxidative Stress in Males
IF: 7.800 -
An herbal drug combination identified by knowledge graph alleviates the clinical symptoms of plasma cell mastitis patients: A nonrandomized controlled trial
IF: 7.700
实验操作视频
