High Sensitivity Human IFN-γ (Interferon Gamma) ELISA Kit
中文名称：高敏型人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.98 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IFN-γ浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.98 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis
IF: 4.996 -
IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway
IF: 4.501Journal： Journal of Oncology (2022)PMID：36213822Reactivity：HumanSample Type：Wilms’ tumor (WT) tissue -
The positive feedback loop of furin and TGFβ1 enhances the immune responses of Tregs to hepatocellular carcinoma cells and hepatitis B virus in vitro
IF: 4.473 -
The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ
IF: 4.345Journal： OncoTargets and Therapy (2022)PMID：30425515Reactivity：HumanSample Type：Cell culture supernatant -
LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway
IF: 4.109 -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657Journal： JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022)PMID：36179036Reactivity：HumanSample Type：fecal -
Possible causes of atherosclerosis: lncRNA COLCA1 induces oxidative stress in human coronary artery endothelial cells and impairs wound healing
IF: 3.616 -
Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function
IF: 3.246 -
A role for serum cytokines and cell adhesion molecules in the non?invasive diagnosis of colorectal cancer
IF: 3.111 -
CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages
IF: 2.596
实验操作视频
