High Sensitivity Human IL-17A (Interleukin 17A) ELISA Kit
中文名称：高敏型人白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 pg/mL
- 灵敏度： 0.49 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-17A浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的人IL-17A会与包被抗体结合。后依次加入生物素化的抗人IL-17A抗体和辣根过氧化物酶标记的亲和素，抗人IL-17A抗体与结合在包被抗体上的人IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 pg/mL |
| 灵敏度 | 0.49 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IL-17A/CEBPβ/OPN/LYVE-1 axis inhibits anti-tumor immunity by promoting tumor-associated tissue-resident macrophages
IF: 7.500 -
Relationship Between Iron Deficiency and Severity of Tuberculosis: Influence on T Cell Subsets
IF: 4.600 -
Profile of immune response during nasal challenge with dermatophagoides pteronyssinus in subjects with allergic airway diseases
IF: 4.400Journal： Journal of Inflammation-London (2024)Reactivity：HumanSample Type：serum,nasal lavage tissue -
Lifestyle deterioration linked to elevated inflammatory cytokines over a two-month follow-up
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：venous blood tissue -
Augmented IFNγ producing ILC1 and IL 17 producing ILC3 in pemphigus vulgaris: Plausible therapeutic target
IF: 3.700Journal： CELLULAR IMMUNOLOGY (2024)Reactivity：HumanSample Type：serum -
Keratinocyte exosomal LOC285194 ameliorates psoriasis by inhibiting the differentiation of CD4+T cells to Th17 cells through regulating miR-211-5p/SIRT1 axis
IF: 3.700 -
Knockdown of PELI1 promotes Th2 and Treg cell differentiation in juvenile idiopathic arthritis
IF: 3.300Journal： EXPERIMENTAL CELL RESEARCH (2024)Reactivity：HumanSample Type：serum -
USP18-mediated protein stabilization of NOTCH1 is associated with altered Th17/Treg cell ratios and B cell-mediated autoantibody secretion in Sj?gren syndrome
IF: 3.300 -
HMGB1 regulates Th17 cell differentiation and function in patients with psoriasis
IF: 3.200Journal： Immunity Inflammation and Disease (2024) -
15-Deoxy-Δ-12,14-Prostaglandin J2 Represses Immune Escape of Lung Adenocarcinoma by Polarizing Macrophages Through Epidermal Growth Factor Receptor/Ras/Raf Pathway
IF: 3.200
实验操作视频
