High Sensitivity Human IL-8 (Interleukin 8) ELISA Kit
中文名称:高敏型人白介素8(IL-8)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 0.47-30 pg/mL
- 灵敏度: 0.29 pg/mL
系列介绍 | Elabscience®采用迭代升级新工艺,自主研发的高敏(High Sensitivity)ELISA试剂盒,聚焦于细胞因子检测,与传统ELISA试剂盒相比,灵敏度提升了2-10倍,可检测fg级别的蛋白浓度,有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-8浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上,实验时样品(或标准品)中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素,抗人IL-8抗体与结合在包被抗体上的人IL-8结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-8浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-8的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.47-30 pg/mL |
灵敏度 | 0.29 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-8,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
H3K4me3-Mediated FOXJ2/SLAMF8 Axis Aggravates Thrombosis and Inflammation in β2GPI/Anti-β2GPI-Treated Monocytes
IF: 14.300 -
Structural identification and anti-stomatitis activity of one arabinose-rich polysaccharide from Rehmannia glutinosa
IF: 7.700Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity:Human -
TLR4 TIR domain and nucleolin GAR domain synergistically mediate RSV infection and induce neuronal inflammatory damage in SH-SY5Y cells
IF: 6.800 -
Helicobacter pylori East Asian type CagA hijacks more SHIP2 by its EPIYA-D motif to potentiate the oncogenicity
IF: 5.500 -
Fabrication and application of targeted ciprofloxacin nanocarriers for the treatment of chronic bacterial prostatitis
IF: 5.200Journal: International Journal of Pharmaceutics-X (2024)Reactivity:HumanSample Type:serum -
Investigating the Anti-Inflammatory Properties and Skin Penetration Ability of Cornelian Cherry (Cornus mas L.) Extracts
IF: 4.900Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity:Human -
Trim31 deficiency exacerbates airway inflammation in asthma by enhancing the activation of the NLRP3 inflammasome
IF: 4.800 -
Role of glucose metabolism reprogramming in keratinocytes in the link between psoriasis and metabolic syndrome
IF: 4.800 -
Effect of Fermented Soybean (FSB) Supplementation on Gastroesophageal Reflux Disease (GERD)
IF: 4.800 -
Role of Increasing Body Mass Index in Gut Barrier Dysfunction, Systemic Inflammation, and Metabolic Dysregulation in Obesity
IF: 4.800