High Sensitivity Mouse IFN-γ (Interferon Gamma) ELISA Kit
中文名称：高敏型小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.84 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IFN-γ浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.84 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Immunization With a Secreted Esterase Protects Mice Against Multiple Serotypes (M1, M3, and M28) of Group A Streptococcus
IF: 4.236 -
Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin
IF: 4.192Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)PMID：32182051Reactivity：Human,MouseSample Type：Serum,Cell culture supernatant -
A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC
IF: 4.187Journal： Microbial Cell Factories (2020)PMID：32075660Reactivity：MouseSample Type：Cell culture supernatant -
Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
IF: 4.092 -
Borneol inhibits CD4?+?T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33272847Reactivity：mouseSample Type：Bronchoalveolar Lavage Fluid -
I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis
IF: 3.758Journal： MEDIATORS OF INFLAMMATION (2020)PMID：32256194Reactivity：MouseSample Type：Serum,Cell culture supernatant,Aqueous Humor -
Comparison of the structural characteristics and immunostimulatory activities of polysaccharides from wild and cultivated Pleurotus feruleus
IF: 3.701Journal： Journal of Functional Foods (2020)Reactivity：MouseSample Type：serum -
Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF?κB activation
IF: 2.914 -
Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection
IF: 2.914Journal： MICROBIAL PATHOGENESIS (2020)PMID：32781100Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum
IF: 1.641
实验操作视频
