High Sensitivity Mouse IFN-γ (Interferon Gamma) ELISA Kit
中文名称：高敏型小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.84 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IFN-γ浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.84 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Cuproptosis in microsatellite stable colon cancer cells affects the cytotoxicity of CD8+T through the WNT signaling pathway
IF: 4.700Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)Reactivity：MouseSample Type：serum -
YTHDF3 phase separation regulates HSPA13-dependent clear cell renal cell carcinoma development and immune evasion
IF: 4.500Journal： CANCER SCIENCE (2024)Reactivity：MouseSample Type：Renca cell-Xenograft -
The correlation between TRIM28 expression and immune checkpoints in CRPC
IF: 4.400Journal： FASEB JOURNAL (2024)Reactivity：MouseSample Type：RM1 cell with TRIM28 knockdown-Xenograft -
Can aged Camellia oleifera Abel oil truly be used to treat atopic dermatitis?
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：serum -
The novel pleuromutilin derivative 22–((4-((4-nitrophenyl)acetamido)phenyl)thio)deoxy pleuromutilin possesses robust anti-mycoplasma activity both in vitro and in vivo
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis
IF: 4.300Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)Reactivity：MouseSample Type：serum -
Evaluation of vaccine candidates against Rhodococcus equi in BALB/c mice infection model: cellular and humoral immune responses
IF: 4.000 -
Disturbance in communication between mitochondrial redox processes and the AMPK/PGC-1α/SIRT-1 axis influences diverse organ symptoms in lupus-affected mice
IF: 3.900 -
miR-155 promotes Th17 differentiation by targeting FOXP3 to aggravate inflammation in MRSA pneumonia
IF: 3.700Journal： CYTOKINE (2024)Reactivity：MouseSample Type：Bronchioalveolar lavage fluid (BALF) -
Rhoifolin Attenuates Concanavalin A-Induced Autoimmune Hepatitis in Mice via JAKs/STATs Mediated Immune and Apoptotic Processes
IF: 3.700
实验操作视频
