High Sensitivity Rat IL-12 (Interleukin 12) ELISA Kit
中文名称：高敏型大鼠白介素12(IL-12)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.63-40 pg/mL
- 灵敏度： 0.42 pg/mL
| 系列介绍 | Elabscience®采用迭代升级新工艺，自主研发的高敏（High Sensitivity）ELISA试剂盒，聚焦于细胞因子检测，与传统ELISA试剂盒相比，灵敏度提升了2-10倍，可检测fg级别的蛋白浓度，有效解决了客户在检测血清、血浆样本时无信号或信号值低的问题。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中IL-12浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-12抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-12会与包被抗体结合。后依次加入生物素化的抗大鼠IL-12抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-12抗体与结合在包被抗体上的大鼠IL-12结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-12浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-12的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.63-40 pg/mL |
| 灵敏度 | 0.42 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-12，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Cang-ai volatile oil alleviates nasal inflammation via Th1/Th2 cell imbalance regulation in a rat model of ovalbumin-induced allergic rhinitis
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：Serum -
The assessment of pharmacokinetics and neuroprotective effect of berberine hydrochloride-embedded albumin nanoparticles via various administration routes: comparative in-vivo studies in rats
IF: 3.000Journal： JOURNAL OF MICROENCAPSULATION (2024)Reactivity：RatSample Type：brain tissue -
Palmatine treats urticaria by reducing inflammation and increasing autophagy
IF: 7.300 -
Combined exposure to manganese and iron decreases oxidative stress-induced nerve damage by increasing Nrf2/HO-1/NQO1 expression
IF: 6.800Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID：38128313Reactivity：RatSample Type：serum -
Salvianolic Acid A Improves Rat Kidney Injury by Regulating MAPKs and TGF-β1/Smads Signaling Pathways
IF: 4.600 -
hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model
IF: 2.200 -
Biomimetic micelles to accurately regulate the inflammatory microenvironment for glomerulonephritis treatment
IF: 10.334 -
Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy
IF: 6.073Journal： Frontiers in Cellular and Infection Microbiology (2022)PMID：35111693Reactivity：RatSample Type：serum,hippocampal tissue -
Exploring the anti-inflammatory potential of Colocasia esculenta root extract in in-vitro and in-vivo models of inflammation
IF: 5.195 -
Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study
IF: 4.657
实验操作视频
