HIS(Histamine) ELISA Kit
中文名称：组胺(HIS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用HIS抗原包被于酶标板上，实验时样品或标准品中的HIS与包被的HIS竞争生物素标记的抗HIS单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，HIS浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中HIS的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的HIS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Screening of probiotic Lactobacilli with potential anti-allergic activity based on hyaluronidase inhibition and degranulation of RBL-2H3 cells in vitro
IF: 4.006Journal： LWT-FOOD SCIENCE AND TECHNOLOGY (2020)Reactivity：RatSample Type：Cell culture supernatant -
Protective Effects of Live Combined B. subtilis and E. faecium in Polymicrobial Sepsis Through Modulating Activation and Transformation of Macrophages and Mast Cells
IF: 3.845 -
Sini San ameliorates duodenal mucosal barrier injury and low?grade inflammation via the CRF pathway in a rat model of functional dyspepsia
IF: 2.928Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019)PMID：31746413Reactivity：RatSample Type：Tissue homogenate -
Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland
IF: 2.535 -
Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice
IF: 2.482 -
In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds
IF: 2.479 -
Coptisine Suppresses Mast Cell Degranulation and Ovalbumin-Induced Allergic Rhinitis
IF: 3.098 -
Anti?allergic effect of Artemisia extract in rats
IF: 1.280Journal： Experimental and Therapeutic Medicine (2016)PMID：27446332Sample Type：Cell culture supernatant
实验操作视频
