Human HMGB-1(High Mobility group protein B1) ELISA Kit

中文名称：人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒

货号：E-EL-H1554
文献（99）
说明书

说明书

别称：

HMG-1、HMG1、HMG3、SBP-1、HMGB1、High mobility group protein B1、High mobility group protein 1 (HMG-1)

Human HMGB-1(High Mobility group protein B1) ELISA Kit

Price: ¥3000.00 ¥2100.00 ¥12750.00

Size:

96 T 48 T 96 T × 5

反应性： Human
检测范围： 31.25-2000 pg/mL
灵敏度： 18.75 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Human
检测方法	比色法;ELISA;夹心法
检测范围	31.25-2000 pg/mL
灵敏度	18.75 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人HMGB-1，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Potential Serum HMGB1, HSP90, and S100A9 as Metastasis Predictive Biomarkers for Cancer Patients and Relevant Cytokines: A Pilot Study

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms252413232

Reactivity：Human

Sample Type：serum
Serum HMGB-1 released by ferroptosis and necroptosis as a novel potential biomarker for systemic lupus erythematosus

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112886

Reactivity：Human

Sample Type：serum
GSDME-mediated pyroptosis promotes anti-tumor immunity of neoadjuvant chemotherapy in breast cancer

IF: 4.6

Journal： CANCER IMMUNOLOGY IMMUNOTHERAPY (2024)

DOI：10.1007/s00262-024-03752-z

Reactivity：Human
GLUT1 mediates the release of HMGB1 from airway epithelial cells in mixed granulocytic asthma

IF: 4.2

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)

DOI：10.1016/j.bbadis.2024.167040

Reactivity：Human

Sample Type：induced sputum
Elevated Serum HMGB1 Levels and Their Association with Recurrence of Acute Ischaemic Stroke

IF: 4.2

Journal： Journal of Inflammation Research (2024)

DOI：10.2147/JIR.S477415

Reactivity：Human

Sample Type：serum
Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis

IF: 3.8

Journal： JOURNAL OF INORGANIC BIOCHEMISTRY (2024)

DOI：10.1016/j.jinorgbio.2024.112610

Reactivity：Human
Overexpression of serum HMGB1 and IDO in esophageal squamous cell carcinoma patients: potential clinical auxiliary diagnostic markers and immunotherapeutic targets

IF: 3.5

Journal： Frontiers in Oncology (2024)

DOI：10.3389/fonc.2024.1452282

Reactivity：Human

Sample Type：serum
TRIM47 promotes HDM-induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF-κB/NLRP3 signaling

IF: 3.3

Journal： CELL BIOLOGY INTERNATIONAL (2024)

DOI：10.1002/cbin.12186

Reactivity：Human
HMGB1 regulates Th17 cell differentiation and function in patients with psoriasis

IF: 3.2

Journal： Immunity Inflammation and Disease (2024)

DOI：10.1002/iid3.1205

PMID：38414294

Reactivity：Human

Sample Type：serum
The Role of High Mobility Group Box B-1 in the Prognosis of Colorectal Cancer Based on the Changes in the Intestinal Mucosal Barrier

IF: 2.8

Journal： TECHNOLOGY IN CANCER RESEARCH & TREATMENT (2024)

DOI：10.1177/15330338231198972

PMID：38200714

Reactivity：Human

Sample Type：serum

Species

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Human

Mouse

Rat

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