Human HMGB-1(High Mobility group protein B1) ELISA Kit
中文名称：人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HMGB-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Effects of AGEs, sRAGE and HMGB1 on Clinical Outcomes in Multiple Myeloma
IF: 0.915Journal： Indian Journal of Hematology and Blood Transfusion (2022)PMID：37006982PMC10064350Reactivity：HumanSample Type：serum -
Synergy of nanodiamond–doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells
IF: 10.435 -
HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism
IF: 7.277 -
CD68+ Macrophage Infiltration Associates With Poor Outcome of HPV Negative Oral Squamous Carcinoma Patients Receiving Radiation: Poly(I:C) Enhances Radiosensitivity of CAL-27 Cells but Promotes Macrophage Recruitment Through HMGB1
IF: 6.244 -
Pharmacological Inhibition of HMGB1 Prevents Muscle Wasting
IF: 5.811Journal： Frontiers in Pharmacology (2021)Reactivity：HumanSample Type：serum -
Extracellular HMGB1 interacts with RAGE and promotes chemoresistance in acute leukemia cells
IF: 5.722 -
In schizophrenia, immune-inflammatory pathways are strongly associated with depressive and anxiety symptoms, which are part of a latent trait which comprises neurocognitive impairments and schizophrenia symptoms.
IF: 4.839 -
Increased Frequency of Circulating Classical Monocytes in Patients with Rosacea
IF: 2.489Journal： Clinical Cosmetic and Investigational Dermatology (2021)Reactivity：HumanSample Type：Plasma -
High mobility group box 1 and homocysteine as preprocedural predictors for contrast-induced acute kidney injury after percutaneous coronary artery intervention
IF: 2.370Journal： INTERNATIONAL UROLOGY AND NEPHROLOGY (2021)PMID：34727314Reactivity：HumanSample Type：serum -
High Mobility Group Protein 1 and Dickkopf-Related Protein 1 in Schizophrenia and Treatment-Resistant Schizophrenia: Associations With Interleukin-6, Symptom Domains, and Neurocognitive Impairments
IF: 7.958
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