Human HMGB-1(High Mobility group protein B1) ELISA Kit
中文名称：人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HMGB-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes
IF: 4.366 -
The association between plasma HMGB1 and sRAGE and clinical outcome in intracerebral hemorrhage
IF: 3.125 -
The Value of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) in Predicting the Severity and Prognosis of Patients After Cardiac Arrest: A Preliminary Observational Study
IF: 2.960 -
Chronic fatigue syndrome and fibromyalgia-like symptoms are an integral component of the phenome of schizophrenia: neuro-immune and opioid system correlates
IF: 2.726 -
Glycyrrhizin suppresses epithelial-mesenchymal transition by inhibiting high-mobility group box1 via the TGF-β1/Smad2/3 pathway in lung epithelial cells
IF: 2.379 -
HMGB-1, TLR4, IL-1R1, TNF-α, and IL-1β: novel epilepsy markers?
IF: 1.276 -
High-mobility Group Box 1 Protein in Pediatric Trauma Patients With Acute Traumatic Coagulopathy or Disseminated Intravascular Coagulation
IF: 1.016Journal： JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY (2020)Reactivity：HumanSample Type：serum -
Doxorubicin conjugated with nanodiamonds and in free form commit glioblastoma cells to heterodromous fates
IF: 4.717 -
Cigarette smoke-induced HMGB1 translocation and release contribute to migration and NF-κB activation through inducing autophagy in lung macrophages
IF: 4.658Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2019)PMID：31769590Reactivity：Human,MouseSample Type：Serum,Cell culture supernatant -
HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas
IF: 3.266Journal： American Journal of Translational Research (2019)DOI：PMID:31934300PMID：31934300Reactivity：HumanSample Type：serum
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