Human HMGB-1(High Mobility group protein B1) ELISA Kit
中文名称：人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上，实验时样品（或标准品）中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素，抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HMGB-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HMGB-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Evidence for SIRT1 Mediated HMGB1 Release From Kidney Cells in the Early Stages of Hemorrhagic Shock
IF: 3.201 -
Role of toll-like receptor 4 in the regulation of the cell death pathway and neuroinflammation
IF: 3.103Journal： BRAIN RESEARCH BULLETIN (2019)PMID：30940475Reactivity：RatSample Type：Cell culture medium -
HMGB1 and SEPP1 as predictors of hepatocellular carcinoma in patients with viral C hepatitis: Effect of DAAs
IF: 2.430 -
Triptolide Suppresses Growth of Breast Cancer by Targeting HMGB1 in Vitro and in Vivo
IF: 1.540Journal： BIOLOGICAL & PHARMACEUTICAL BULLETIN (2019)PMID：30956264Reactivity：HumanSample Type：Cell culture supernatant -
Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity
IF: 1.121Journal： Genetic Testing and Molecular Biomarkers (2019)PMID：30562142Reactivity：HumanSample Type：Plasma -
Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma
IF: 8.806 -
Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing–Remitting-Multiple Sclerosis Patients
IF: 5.511 -
29-kDa FN-f inhibited autophagy through modulating localization of HMGB1 in human articular chondrocytes
IF: 3.085 -
Recombined humanized endostatin-induced suppression of HMGB1 expression inhibits proliferation of NSCLC cancer cells
IF: 2.569 -
Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment
IF: 7.786Journal： JOURNAL OF CONTROLLED RELEASE (2017)PMID：29051064Reactivity：HumanSample Type：Cell culture supernatant
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