Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-EL-H0109
文献（636）
说明书

说明书

别称：

TNF、DIF、TNF-alpha、TNFA、TNFSF2、TNLG1F、TNF alpha

Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Human
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的人TNF-α会与包被抗体结合。后依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素，抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Human
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

IF: 6.530

Journal： BIOMEDICINE & PHARMACOTHERAPY (2021)

DOI：10.1016/j.biopha.2021.111531

PMID：34311530

Reactivity：Human,Mouse

Sample Type：Serum
Changes in genotoxicity, inflammatory and oxidative stress parameters of workers in marble processing plants

IF: 6.498

Journal： ENVIRONMENTAL RESEARCH (2021)

DOI：10.1016/j.envres.2021.111209

PMID：33895109

Reactivity：Human

Sample Type：Plasma
Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

IF: 6.313

Journal： Antioxidants (2021)

DOI：10.3390/antiox10111741

PMID：34829612

Reactivity：Human

Sample Type：saliva
miR-122–5p regulates hepatocytes damage caused by BaP and DBP co-exposure through SOCS1/STAT3 signaling in vitro

IF: 6.291

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)

DOI：10.1016/j.ecoenv.2021.112570

PMID：34352581

Reactivity：Human
Effects of delta-tocotrienol supplementation on Glycemic Control, oxidative stress, inflammatory biomarkers and miRNA expression in type 2 diabetes mellitus: A randomized control trial

IF: 5.882

Journal： PHYTOTHERAPY RESEARCH (2021)

DOI：10.1002/ptr.7113

PMID：33899292

Reactivity：Human

Sample Type：Serum
Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

IF: 5.742

Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2021)

DOI：10.3748/wjg.v27.i38.6489

PMID：34720537

Reactivity：Human

Sample Type：serum
Downregulation of p300/CBP-associated factor inhibits cardiomyocyte apoptosis via suppression of NF-κB pathway in ischaemia/reperfusion injury rats

IF: 5.310

Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)

DOI：10.1111/jcmm.16959

PMID：34601814

Reactivity：Rat

Sample Type：myocardial tissue,blood
Normothermic machine perfusion attenuates hepatic ischaemia-reperfusion injury by inhibiting CIRP-mediated oxidative stress and mitochondrial fission

IF: 5.310

Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)

DOI：10.1111/jcmm.17062

PMID：34786826

Reactivity：Human

Sample Type：serum
GLUT3 as an Intersection of Glycerophospholipid Metabolism and the Innate Immune Response to Candida albicans

IF: 5.293

Journal： Frontiers in Cellular and Infection Microbiology (2021)

DOI：10.3389/fcimb.2021.648988

PMID：34222036

Reactivity：Human
Critically Ill Coronavirus Disease 2019 Patients Exhibit Hyperactive Cytokine Responses Associated With Effector Exhausted Senescent T Cells in Acute Infection

IF: 5.226

Journal： JOURNAL OF INFECTIOUS DISEASES (2021)

DOI：10.1093/infdis/jiab425

Reactivity：Human

Sample Type：serum

Species

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Canine

Human

Mouse

Porcine

Rat

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