Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-EL-H0109
文献（636）
说明书

说明书

别称：

TNF、DIF、TNF-alpha、TNFA、TNFSF2、TNLG1F、TNF alpha

Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Human
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的人TNF-α会与包被抗体结合。后依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素，抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Human
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

The association between a metabolically healthy overweight/obesity phenotype and markers of inflammation among Chinese children and adolescents aged 10–18 years

IF: 1.634

Journal： JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM (2021)

DOI：10.1515/jpem-2021-0224

Reactivity：Human

Sample Type：serum
Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

IF: 1.538

Journal： ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery (2021)

DOI：10.1159/000511711

PMID：33395682

Reactivity：Human

Sample Type：serum
Comparison of Inflammation Biomarkers among Chronic Obstructive Pulmonary Disease Groups: A Cross Sectional Study

IF: 0.968

Journal： Nigerian Journal of Clinical Practice (2021)

DOI：10.4103/njcp.njcp_222_19

Reactivity：Human

Sample Type：serum
Elevated Levels of Activin-A, TNF-Alpha and IL-6 in Acromegaly

IF: 0.480

Journal： Neurochemical Journal (2021)

DOI：10.1134/S181971242103003X

Reactivity：Human

Sample Type：serum
Oxidative stress index can be a new marker related to disease severity in COVID-19

IF: 0.401

Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2021)

DOI：10.1515/tjb-2021-0013

Reactivity：Human

Sample Type：serum
The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

Journal： Journal of Blood Medicine (2021)

DOI：10.2147/JBM.S305566

PMID：34267571

Reactivity：Human
Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

IF: 38.637

Journal： CELL (2020)

DOI：10.1016/j.cell.2020.08.009

PMID：32931733

Reactivity：Human

Sample Type：Cell culture medium
MicroRNA-101-3p Downregulates TLR2 Expression, Leading to Reduction in Cytokine Production by Treponema pallidum–Stimulated Macrophages

IF: 7.143

Journal： JOURNAL OF INVESTIGATIVE DERMATOLOGY (2020)

DOI：10.1016/j.jid.2019.12.012

PMID：31930972

Reactivity：Human

Sample Type：plasma
MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma

IF: 7.068

Journal： JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2020)

DOI：10.1186/s13046-020-01752-2

PMID：33168024

Reactivity：Human

Sample Type：Cell culture supernatant
Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

IF: 5.546

Journal： JOURNAL OF CELLULAR PHYSIOLOGY (2020)

DOI：10.1002/jcp.29713

PMID：32385929

Reactivity：Human

Sample Type：Cell culture supernatant

Species

{{relatedElisaPage.numTotal}} Results

All

Canine

Human

Mouse

Porcine

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual

Cat.No.：{{item.cat}}

{{index}}{{goods_show_value}}