Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称：人血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人VEGF-A抗体包被于酶标板上，实验时样品（或标准品）中的人VEGF-A会与包被抗体结合。后依次加入生物素化的抗人VEGF-A抗体和辣根过氧化物酶标记的亲和素，抗人VEGF-A抗体与结合在包被抗体上的人VEGF-A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VEGF-A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人VEGF-A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients
IF: 2.145Journal： International Journal of General Medicine (2022)PMID：28008280Reactivity：HumanSample Type：Plasma -
Effects of acute aerobic exercise on cytokines, klotho, irisin, and vascular endothelial growth factor responses in rheumatoid arthritis patients
IF: 2.089Journal： IRISH JOURNAL OF MEDICAL SCIENCE (2022)Reactivity：HumanSample Type：sera -
An observational study of pro- and anti-angiogenic factors in hypertensive disorders of pregnancy in women of African ancestry
IF: 1.226Journal： JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2022)Reactivity：HumanSample Type：serum -
The effects of vitamin E on non-proliferative diabetic retinopathy in type 2 diabetes mellitus: Are they sustainable with 12?months of therapy
-
Sevoflurane preconditioning promotes mesenchymal stem cells to relieve myocardial ischemia/reperfusion injury via TRPC6-induced angiogenesis
IF: 6.832 -
Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma
IF: 6.244 -
LncRNA MALAT1 Promotes Tumor Angiogenesis by Regulating MicroRNA-150-5p/VEGFA Signaling in Osteosarcoma: In-Vitro and In-Vivo Analyses
IF: 6.244 -
A Phase IIb Randomized Controlled Trial Investigating the Effects of Tocotrienol-Rich Vitamin E on Diabetic Kidney Disease
IF: 5.719 -
Tocotrienol-Rich Vitamin E (Tocovid) Improved Nerve Conduction Velocity in Type 2 Diabetes Mellitus Patients in a Phase II Double-Blind, Randomized Controlled Clinical Trial
IF: 5.719 -
MiR-195-5p and miR-205-5p in extracellular vesicles isolated from diabetic foot ulcer wound fluid decrease angiogenesis by inhibiting VEGFA expression
IF: 5.682
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
