Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称：人血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人VEGF-A抗体包被于酶标板上，实验时样品（或标准品）中的人VEGF-A会与包被抗体结合。后依次加入生物素化的抗人VEGF-A抗体和辣根过氧化物酶标记的亲和素，抗人VEGF-A抗体与结合在包被抗体上的人VEGF-A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VEGF-A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人VEGF-A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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IF: 1.0Journal： Cureus Journal of Medical Science (2024)Reactivity：HumanSample Type：serum -
Injectable engineered micro/nano-complexes trigger the reprogramming of bone immune epigenetics
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Carbon dots efficiently promote vascularization for enhanced repairing of orthopedic diseases with diabetic mellitus based on nanocatalytic medicine
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Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure
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Metformin promotes angiogenesis by enhancing VEGFa secretion by adipose-derived stem cells via the autophagy pathway
IF: 6.700 -
dCas9-Based PDGFR–β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：36983022Reactivity：HumanSample Type：adipose tissue (ADSCs) -
Serum VEGF and CGRP Biomarkers: Relationships with Pain Intensity, Electric Pain, Pressure Pain Threshold, and Clinical Symptoms in Fibromyalgia—An Observational Study
IF: 5.6Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：37958517Reactivity：HumanSample Type：serum -
Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome
IF: 5.2Journal： Frontiers in Endocrinology (2023)PMID：37670876Reactivity：HumanSample Type：ovarian tissue -
ITGA5 promotes tumor angiogenesis in cervical cancer
IF: 4.000
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