Human ACE2(Angiotensin Ⅰ Converting Enzyme 2) ELISA Kit
中文名称：人血管紧张素转化酶2(ACE2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.39-25 ng/mL
- 灵敏度： 0.23 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ACE2抗体包被于酶标板上，实验时样品（或标准品）中的人ACE2会与包被抗体结合。后依次加入生物素化的抗人ACE2抗体和辣根过氧化物酶标记的亲和素，抗人ACE2抗体与结合在包被抗体上的人ACE2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ACE2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ACE2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人ACE2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
ACE2 deficiency inhibits thoracic aortic dissection by enhancing SIRT3 mediated inhibition of inflammation and VSCMs phenotypic switch
IF: 6.000Journal： MOLECULAR MEDICINE (2024)Reactivity：HumanSample Type：serum -
A longitudinal study on the effect of obesity upon circulating renin angiotensin system in normal pregnancy
IF: 3.900Journal： NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (2023)PMID：38161127Reactivity：HumanSample Type：Blood -
Chemotherapy induces ACE2 expression in breast cancer via the ROS-AKT-HIF-1α signaling pathway: a potential prognostic marker for breast cancer patients receiving chemotherapy
IF: 8.440 -
Type 1 diabetes is associated with significant changes of ACE and ACE2 expression in peripheral blood mononuclear cells
IF: 4.666 -
The Predictive Value of Serum ACE2 and TMPRSS2 Concentrations in Patients with COVID-19—A Prospective Pilot Study
IF: 3.508 -
HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism
IF: 7.277 -
Antcins from Antrodia cinnamomea and Antrodia salmonea Inhibit Angiotensin-Converting Enzyme 2 (ACE2) in Epithelial Cells: Can Be Potential Candidates for the Development of SARS-CoV-2 Prophylactic Agents
IF: 3.935 -
Sex Differences in Proatherogenic Cytokine Levels
IF: 4.556Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)PMID：32485823Reactivity：HumanSample Type：Serum -
Geranium and Lemon Essential Oils and Their Active Compounds Downregulate Angiotensin-Converting Enzyme 2 (ACE2), a SARS-CoV-2 Spike Receptor-Binding Domain, in Epithelial Cells
IF: 2.762
实验操作视频
