Human ACE(Angiotensin Ⅰ Converting Enzyme) ELISA Kit
中文名称：人血管紧张素转化酶(ACE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ACE抗体包被于酶标板上，实验时样品（或标准品）中的人ACE会与包被抗体结合。后依次加入生物素化的抗人ACE抗体和辣根过氧化物酶标记的亲和素，抗人ACE抗体与结合在包被抗体上的人ACE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ACE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ACE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人ACE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Angiotensin II Type I Receptor—168A/G Polymorphism Is Associated with Increased the Risk of Glioma in Turkish Population
IF: 1.200Journal： MOLECULAR BIOLOGY (2024)Reactivity：HumanSample Type：Serum,glial tumors -
A longitudinal study on the effect of obesity upon circulating renin angiotensin system in normal pregnancy
IF: 3.900Journal： NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES (2023)PMID：38161127Reactivity：HumanSample Type：Blood -
Angiotensin-Converting Enzyme (ACE) level, but not ACE gene polymorphism, is associated with prognosis of COVID-19 infection: Implications for diabetes and hypertension
IF: 3.700 -
Association polymorphism of guanine nucleotide–binding protein β3 subunit (GNB3) C825T and insertion/deletion of the angiotensin-converting enzyme (ACE) gene with peripartum cardiomyopathy
IF: 3.600Journal： Frontiers in Cardiovascular Medicine (2023)PMID：37089887Reactivity：HumanSample Type：Plasma -
The Effect of Angiotensin Converting Enzyme (ACE) I/D Polymorphism on Atherosclerotic Cardiovascular Disease and Cardiovascular Mortality Risk in Non-Hemodialyzed Chronic Kidney Disease: The Mediating Role of Plasma ACE Level
IF: 4.141 -
Pulmonary midkine inhibition ameliorates sepsis induced lung injury
IF: 5.531
实验操作视频
