Human ANP(Atrial Natriuretic Peptide) ELISA Kit
中文名称：人心钠肽(ANP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用人ANP抗原包被于酶标板上，实验时样品或标准品中的人ANP与包被的人ANP竞争生物素标记的抗人ANP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，人ANP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中人ANP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人ANP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Evaluation of some nonroutine cardiac biomarkers among adults and children with beta-thalassemia major
IF: 1.300 -
Low-dose of Polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids
IF: 11.800Journal： ENVIRONMENT INTERNATIONAL (2023)PMID：37669592Reactivity：HumanSample Type：cardiac organoids (COs) -
Role of atrial natriuretic peptide in the dissociation between flow relations with ventricular mass and function in a community with volume-dependent hypertension
IF: 3.600Journal： Frontiers in Cardiovascular Medicine (2023)PMID：37265568Reactivity：HumanSample Type：plasma -
Atrial Fibrillation in Heart Failure: Epidemiology, Quality of Life and Clinical Characteristics in the Iron Deficiency and ANaemia in Heart Failure (IDAN-HF) Study in Ogbomoso, Nigeria
IF: 1.200 -
Attenuated Relationships Between Indexes of Volume Overload and Atrial Natriuretic Peptide in Uncontrolled, Sustained Volume-Dependent Primary Hypertension
IF: 9.897 -
Atrial natriuretic peptide and posterior pituitary neurohormone changes in patients with acute schizophrenia
IF: 2.989Journal： Neuropsychiatric Disease and Treatment (2022)PMID：30038496Reactivity：HumanSample Type：Serum -
ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats
IF: 5.965 -
Raised HIF1α during normoxia in high altitude pulmonary edema susceptible non-mountaineers
IF: 5.228
实验操作视频
