Human ApoA4(Apolipoprotein A4) ELISA Kit
中文名称：人载脂蛋白A4(ApoA4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 4.69-300 ng/mL
- 灵敏度： 2.81 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人ApoA4抗体包被于酶标板上，实验时样品（或标准品）中的人ApoA4会与包被抗体结合。后依次加入生物素化的抗人ApoA4抗体和辣根过氧化物酶标记的亲和素，抗人ApoA4抗体与结合在包被抗体上的人ApoA4结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ApoA4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ApoA4的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 4.69-300 ng/mL |
| 灵敏度 | 2.81 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人ApoA4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal
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Bioinformatic Analysis of the Proteome in Exosomes Derived From Plasma: Exosomes Involved in Cholesterol Metabolism Process of Patients With Spinal Cord Injury in the Acute Phase
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A novel electrochemical immunosensor for the highly sensitive and selective detection of the depression marker human apolipoprotein A4
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Identifying functional non-coding variants in APOA5/A4/C3/A1 gene cluster associated with coronary heart disease
IF: 4.133Journal： JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2020)PMID：32437778Reactivity：HumanSample Type：Serum -
iTRAQ-based proteomic analysis of plasma reveals abnormalities in lipid metabolism proteins in chronic kidney disease-related atherosclerosis
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Label-Free Quantitative Proteomics Reveals Differences in Molecular Mechanism of Atherosclerosis Related and Non-Related to Chronic Kidney Disease
IF: 3.257Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016)PMID：27144566Reactivity：HumanSample Type：Plasma
实验操作视频
