Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称：人脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人BDNF抗体包被于酶标板上，实验时样品（或标准品）中的人BDNF会与包被抗体结合。后依次加入生物素化的抗人BDNF抗体和辣根过氧化物酶标记的亲和素，抗人BDNF抗体与结合在包被抗体上的人BDNF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，BDNF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中BDNF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人BDNF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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An analysis of the relationship of “the Mozart effect” with BDNF levels in anatomy education
IF: 7.300Journal： Anatomical Sciences Education (2024)DOI：10.1002/ase.2409PMID：38509019Reactivity：HumanSample Type：plasma -
Cognitive functions and the brain-derived neurotrophic factor in patients with MACS
IF: 5.300Journal： EUROPEAN JOURNAL OF ENDOCRINOLOGY (2024)Reactivity：HumanSample Type：Serum -
Association between cognitive function and skeletal muscle in patients undergoing maintenance hemodialysis
IF: 5.200 -
Optimizing esketamine administration for postoperative depression: a comprehensive study on laparoscopic bariatric surgery patients
IF: 3.500Journal： PSYCHOPHARMACOLOGY (2024)Reactivity：HumanSample Type：Plasma -
Preclinical evaluation on human platelet lysate for the treatment of secondary injury following intracerebral hemorrhage
IF: 3.500Journal： BRAIN RESEARCH BULLETIN (2024)Reactivity：HumanSample Type：Human platelet lysate (HPL) -
Exploring the Influence of IL-8, IL-10, Patient-Reported Pain, and Physical Activity on Endometriosis Severity
IF: 3.000 -
Unique content of breastmilk: neurotrophic growth factors in breastmilk at 2 years and beyond
IF: 3.000Journal： EUROPEAN JOURNAL OF PEDIATRICS (2024)Reactivity：HumanSample Type：breast milk sample -
Impact of Acute High-intensity Interval Training on Cortical Excitability, M1-related Cognitive Functions, and Myokines: A Randomized Crossover Study
IF: 2.900Journal： NEUROSCIENCE (2024)Reactivity：HumanSample Type：serum -
Levels of biomarkers associated with subconcussive head hits in mixed martial arts fighters
IF: 2.300 -
UP150 Project: A Longitudinal Analysis of Active Lifestyles in the Complex Working System
IF: 2.200
实验操作视频
