Human C3(Complement Component 3) ELISA Kit
中文名称：人补体成分3(C3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人C3抗体包被于酶标板上，实验时样品（或标准品）中的人C3会与包被抗体结合。后依次加入生物素化的抗人C3抗体和辣根过氧化物酶标记的亲和素，抗人C3抗体与结合在包被抗体上的人C3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，C3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中C3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人C3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
One-Year Outcomes for Depression and Anxiety in SLE Patients
IF: 4.700 -
Comparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in the Eyes of High Myopic Patients
IF: 4.400Journal： JOURNAL OF PROTEOME RESEARCH (2024)PMID：38367214Reactivity：HumanSample Type：aqueous humor -
Pleural Mesothelial Cells-Induced Monocytes to the Pleural Cavity through the Effect of C3 Lytic Products in Tuberculous Pleural Effusion
IF: 2.600Journal： INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2024)Reactivity：HumanSample Type：pleural fluids,plasma -
Immune-dysregulation in subacute sclerosing panencephalitis: An exploratory case–control study
IF: 12.700 -
Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction
IF: 11.100 -
Astrocyte-derived complement C3 is activated in patients with tuberous sclerosis complex and mediates immune injury: an integrated bioinformatics analysis
IF: 2.000 -
Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets
IF: 6.590 -
A Study of IFN-α-Induced Chemokines CCL2, CXCL10 and CCL19 in Patients with Systemic Lupus Erythematosu
IF: 3.251 -
Prevalence of primary immunodeficiency syndromes in tuberculous meningitis: A case-control study
IF: 3.718Journal： Journal of Infection and Public Health (2021)PMID：34883295Reactivity：HumanSample Type：serum -
Altered levels of complement components associated with non-immediate drug hypersensitivity reactions
IF: 1.734
实验操作视频
