Human C5a(Complement Component 5a) ELISA Kit
中文名称：人补体成分5a(C5a)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人C5a抗体包被于酶标板上，实验时样品（或标准品）中的人C5a会与包被抗体结合。后依次加入生物素化的抗人C5a抗体和辣根过氧化物酶标记的亲和素，抗人C5a抗体与结合在包被抗体上的人C5a结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，C5a浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中C5a的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人C5a，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Pleural Mesothelial Cells-Induced Monocytes to the Pleural Cavity through the Effect of C3 Lytic Products in Tuberculous Pleural Effusion
IF: 2.600Journal： INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2024)Reactivity：HumanSample Type：pleural fluids,plasma -
Immune modulation as a consequence of SARS-CoV-2 infection.
IF: 8.786 -
miR-132-3p regulates antibody-mediated complement-dependent cytotoxicity in colon cancer cells by directly targeting CD55
IF: 5.732 -
By Increasing the Expression and Activation of STAT3, Sustained C5a Stimulation Increases the Proliferation, Migration, and Invasion of RCC Cells and Promotes the Growth of Transgrafted Tumors
IF: 3.602 -
Recruitment of IL-1β-producing intermediate monocytes enhanced by C5a contributes to the development of malignant pleural effusion
IF: 3.223Journal： Thoracic Cancer (2022)Reactivity：HumanSample Type：pleural effusion -
Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion
IF: 4.411Journal： PLoS Neglected Tropical Diseases (2021)PMID：34237073Reactivity：HumanSample Type：pleural fluid -
CD55 limits sensitivity to complement-dependent cytolysis triggered by heterologous expression of α-gal xenoantigen in colon tumor cells
IF: 3.737Journal： AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY (2014)PMID：24763553Reactivity：Human
实验操作视频
