Human CALP(Calprotectin) ELISA Kit
中文名称：人钙卫蛋白(CALP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人CALP抗体包被于酶标板上，实验时样品（或标准品）中的人CALP会与包被抗体结合。后依次加入生物素化的抗人CALP抗体和辣根过氧化物酶标记的亲和素，抗人CALP抗体与结合在包被抗体上的人CALP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CALP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CALP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人CALP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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PD-L1+ neutrophils induced NETs in malignant ascites is a potential biomarker in HCC
IF: 4.600Journal： CANCER IMMUNOLOGY IMMUNOTHERAPY (2024)Reactivity：HumanSample Type：serum,ascite tissue -
A distinct microbiota signature precedes the clinical diagnosis of hepatocellular carcinoma
IF: 12.200 -
LP-004?Serum calprotectin could be a novel marker of microangiopathy in antiphospholipid syndrome patients
IF: 3.900Journal： Lupus Science & Medicine (2023)Reactivity：HumanSample Type：serum -
Calprotectin and periostin levels in periodontitis patients with coronary artery disease
IF: 3.800 -
Serum Calprotectin as a Potential Predictor of Microvascular Manifestations in Patients with Antiphospholipid Syndrome
IF: 3.800 -
The use of calprotectin and other inflammatory parameters in the investigation of pseudoexfoliation syndrome concomitant glaucoma and systemic diseases
IF: 3.100 -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657Journal： JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022)PMID：36179036Reactivity：HumanSample Type：fecal -
Peritoneal calprotectin level in peritoneal dialysis patients
IF: 2.886Journal： SEMINARS IN DIALYSIS (2022)PMID：35439838Reactivity：HumanSample Type：peritoneal dialysate drainage fluid -
Serum calprotectin levels as markers of inflammation, insulin resistance and hyperandrogenism in women with polycystic ovary syndrome
IF: 1.817 -
Chronic Pulmonary Aspergillosis: Disease Severity Using Image Analysis and Correlation with Systemic Proinflammation and Predictors of Clinical Outcome
IF: 5.816
实验操作视频
