Human CASP1(Caspase 1) ELISA Kit
中文名称：人胱天蛋白酶1(CASP1)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人CASP1抗体包被于酶标板上，实验时样品（或标准品）中的人CASP1会与包被抗体结合。后依次加入生物素化的抗人CASP1抗体和辣根过氧化物酶标记的亲和素，抗人CASP1抗体与结合在包被抗体上的人CASP1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人CASP1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Protective role of forsythoside B in Kawasaki disease-induced cardiac injury: Inhibition of pyroptosis via the SIRT1–NF-κB–p65 signaling pathway
IF: 5.100Evaluation of inflammasomes as biomarker following non-surgical periodontal treatment
IF: 2.200Journal： ARCHIVES OF ORAL BIOLOGY (2024)Reactivity：HumanSample Type：saliva ,serumMelatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression
IF: 6.700Regulatory Ability of Lactiplantibacillus plantarum on Human Skin Health by Counteracting In Vitro Malassezia furfur Effects
IF: 4.700A comparative study of modulatory interaction between cytokines and apoptotic proteins among Scleroderma patients with and without pulmonary involvement
IF: 3.800Nanoparticle Emulsions Enhance the Inhibition of NLRP3
IF: 6.208Periodontitis activates the NLRP3 inflammasome in serum and saliva
IF: 6.993Association between serum NLRP3 and malignant brain edema in patients with acute ischemic stroke
IF: 2.474Salivary Levels of NLRP3 Inflammasome-Related Proteins as Potential Biomarkers of Periodontal Clinical Status
IF: 3.030
