Human CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
中文名称:人肌酸激酶同工酶MB(CKMB)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 7.81-500 ng/mL
- 灵敏度: 4.69 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人CKMB抗体包被于酶标板上,实验时样品(或标准品)中的人CKMB会与包被抗体结合。后依次加入生物素化的抗人CKMB抗体和辣根过氧化物酶标记的亲和素,抗人CKMB抗体与结合在包被抗体上的人CKMB结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CKMB浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CKMB的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 ng/mL |
灵敏度 | 4.69 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人CKMB,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Long noncoding RNA NONHSAT122636.2 attenuates myocardial inflammation and apoptosis in myocarditis
IF: 2.900 -
Enhanced therapeutic efficacy of doxorubicin against multidrug-resistant breast cancer with reduced cardiotoxicity
IF: 6.000 -
Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression
IF: 8.469Journal: Cell Death & Disease (2021)PMID:33986259Reactivity:HumanSample Type:Cell culture supernatant -
The effect of coenzyme Q10 plus trimetazidine on acute viral myocarditis treatment
IF: 4.060Journal: American Journal of Translational Research (2021)DOI:PMID:35035725PMID:35035725Reactivity:HumanSample Type:serum -
Downregulation of P300/CBP-Associated Factor Attenuates Myocardial Ischemia-Reperfusion Injury Via Inhibiting Autophagy
IF: 2.523Journal: International Journal of Medical Sciences (2020)PMID:32547315Reactivity:HumanSample Type:Serum,Cell culture supernatant -
Association between carbonyl stress markers and the risk of acute coronary syndrome in patients with type 2 diabetes mellitus–A pilot study
Journal: Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2020)Reactivity:HumanSample Type:serum -
Circulating Long Noncoding RNA LIPCAR Acts as a Novel Biomarker in Patients with ST-Segment Elevation Myocardial Infarction
IF: 1.894