Human COMP(Cartilage Oligomeric Matrix Protein) ELISA Kit
中文名称:人软骨寡聚基质蛋白(COMP)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Human
- 检测范围: 0.63-40 ng/mL
- 灵敏度: 0.38 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人COMP抗体包被于酶标板上,实验时样品(或标准品)中的人COMP会与包被抗体结合。后依次加入生物素化的抗人COMP抗体和辣根过氧化物酶标记的亲和素,抗人COMP抗体与结合在包被抗体上的人COMP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,COMP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中COMP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.63-40 ng/mL |
灵敏度 | 0.38 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人COMP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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IF: 4.700Development of Cissus quadrangularis-Loaded POSS-Reinforced Chitosan-Based Bilayer Sponges for Wound Healing Applications: Drug Release and In Vitro Bioactivity
IF: 4.100Effectiveness of a Cucumber Extract Supplement on Articular Pain in Patients with Knee Osteoarthritis: A Randomized Double-Blind Controlled Clinical Trial
IF: 2.838Bioactive snail mucus-slime extract loaded chitosan scaffolds for hard tissue regeneration: the effect of mucoadhesive and antibacterial extracts on physical characteristics and bioactivity of chitosan matrix
IF: 3.715Short- and Long-Term Effectiveness of Supplementation with Non-Animal Chondroitin Sulphate on Inflammation, Oxidative Stress and Functional Status in Obese Subjects with Moderate Knee Osteoarthritis before and after Physical Stress: A Randomized, Double-Blind, Placebo-Controlled Trial
IF: 5.014Elevated serum cartilage oligomeric matrix protein and the metalloproteinase-ADAMTS7 levels are associated with vascular calcification in maintenance hemodialysis patients
IF: 2.164Joint remodeling outcome of serum levels of Dickkopf-1 (DKK1), cartilage oligomeric matrix protein (COMP), and C-telopeptide of type II collagen (CTXII) in rheumatoid arthritis
IF: 1.415Journal: Central European Journal of Immunology (2020)PMID:32425683Reactivity:HumanSample Type:SerumAssociations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis
IF: 2.293Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis
IF: 4.742