Human CRP(C-Reactive Protein) ELISA Kit
中文名称：人C反应蛋白(CRP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.39-25 ng/mL
- 灵敏度： 0.23 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人CRP抗体包被于酶标板上，实验时样品（或标准品）中的人CRP会与包被抗体结合。后依次加入生物素化的抗人CRP抗体和辣根过氧化物酶标记的亲和素，抗人CRP抗体与结合在包被抗体上的人CRP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CRP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CRP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人CRP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis
IF: 5.700Journal： Frontiers in Immunology (2024)Reactivity：HumanSample Type：plasma -
Role of Oxidative Stress and Inflammation in Postoperative Complications and Quality of Life After Laryngeal Cancer Surgery
IF: 5.100 -
Vitamin D3 improves glucose metabolism and attenuates inflammation in prediabetic human and mice
IF: 4.800Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)Reactivity：HumanSample Type：serum -
Iron absorption in adults with sickle cell anemia: a stable-isotope approach
IF: 4.100Journal： EUROPEAN JOURNAL OF NUTRITION (2024)Reactivity：HumanSample Type：Serum -
Lifestyle deterioration linked to elevated inflammatory cytokines over a two-month follow-up
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：venous blood tissue -
Composition of gut microbiota in obese and normal-weight Uygur adults and its association with adipocyte-related factors
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：Serum -
The FGF23–Klotho axis promotes microinflammation in chronic kidney disease
IF: 3.700 -
Iron incorporation in red blood cells of pediatric sickle cell anemia: a stable isotope pilot investigation
IF: 3.600Journal： EUROPEAN JOURNAL OF CLINICAL NUTRITION (2024)Reactivity：HumanSample Type：Serum -
Whole-Genome Metagenomic Analysis of the Oral Microbiota in Patients with Obstructive Sleep Apnea Comorbid with Major Depressive Disorder
IF: 3.000Journal： Nature and Science of Sleep (2024)Reactivity：HumanSample Type：Plasma -
Antioxidant Potential, DNA Damage, Inflammation, Glycemic Control and Lipid Metabolism Alteration: A Mediation Analysis of Islamic Sunnah Intermittent Fasting on Cognitive Function among Older Adults with Mild Cognitive Impairment
IF: 5.285Journal： Journal of Nutrition Health & Aging (2022)Reactivity：HumanSample Type：plasma
实验操作视频
