Human Cyt-C(Cytochrome C) ELISA Kit
中文名称：人细胞色素C(Cyt-C)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人Cyt-C抗体包被于酶标板上，实验时样品（或标准品）中的人Cyt-C会与包被抗体结合。后依次加入生物素化的抗人Cyt-C抗体和辣根过氧化物酶标记的亲和素，抗人Cyt-C抗体与结合在包被抗体上的人Cyt-C结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Cyt-C浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Cyt-C的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人Cyt-C，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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α-Mangostin-phytosomes as a plausible nano-vesicular approach for enhancing cytotoxic activity on SKOV-3 ovarian cancer cells
IF: 3.400Journal： Future Journal of Pharmaceutical Sciences (2024)Reactivity：Human -
The recovery from taxane mediated apoptosis in PC-3 castration-resistant metastatic prostate cancer cells
IF: 2.600 -
Optimized bilosome-based nanoparticles enhance cytotoxic and pro-apoptotic activity of costunolide in LS174T colon cancer cells
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Repurposing synthetic and natural derivatives induces apoptosis in an orthotopic glioma-induced xenograft model by modulating WNT/β-catenin signaling
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High-fat diet alters intestinal microbiota and induces endoplasmic reticulum stress via the activation of apoptosis and inflammation in blunt snout bream
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Response of Circulating Inflammatory Markers to Intermittent Hypoxia-Hyperoxia Training in Healthy Elderly People and Patients with Mild Cognitive Impairment
IF: 3.251 -
Altered expression of ERK, Cytochrome-c, and HSP70 triggers apoptosis in Quinacrine-exposed human invasive ductal carcinoma cells
IF: 6.530Journal： BIOMEDICINE & PHARMACOTHERAPY (2021)PMID：34243615Reactivity：HumanSample Type：Cell lysate -
Chronic inflammation is a key to inducing liver injury in blunt snout bream (Megalobrama amblycephala) fed with high-fat diet
IF: 3.119Journal： DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY (2019)PMID：30910418Reactivity：FishSample Type：Tissue homogenate -
Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A
IF: 1.538
Q1:Cytochrome-C，Cytochrome P450 aromatase 这两个指标有什么不同？
Cytochrome-C：最丰富、最稳定的细胞色素,主要参与能量转移；
Cytochrome P450 aromatase ：一种细胞色素P450单加氧酶，催化C19雄激素、雄激素-4-烯-3,17-二酮(雄烯二酮)和睾酮分别转化为C18雌激素、雌酮和雌二醇。
实验操作视频
