Human DKK1(Dickkopf Related Protein 1) ELISA Kit
中文名称：人Dickkopf相关蛋白1(DKK1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人DKK1抗体包被于酶标板上，实验时样品（或标准品）中的人DKK1会与包被抗体结合。后依次加入生物素化的抗人DKK1抗体和辣根过氧化物酶标记的亲和素，抗人DKK1抗体与结合在包被抗体上的人DKK1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，DKK1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中DKK1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人DKK1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Changes in Dickkopf-1, but Not Sclerostin, in Gingival Crevicular Fluid Are Associated with Peroral Statin Treatment in Patients with Periodontitis
IF: 2.600Journal： Medicina-Lithuania (2024)PMID：38541234Reactivity：HumanSample Type：Gingival Crevicular Fluid (GCF) -
Relationship of Dickkopf-1 With Atherosclerosis and Arterial Stiffness in Renal Transplant Recipients
IF: 0.800Journal： TRANSPLANTATION PROCEEDINGS (2024)Reactivity：HumanSample Type：Serum -
Impact of REAC Regenerative Endogenous Bioelectrical Cell Reprogramming on MCF7 Breast Cancer Cells
IF: 3.508 -
In schizophrenia, immune-inflammatory pathways are strongly associated with depressive and anxiety symptoms, which are part of a latent trait which comprises neurocognitive impairments and schizophrenia symptoms.
IF: 4.839 -
In schizophrenia, non-remitters and partial remitters to treatment with antipsychotics are qualitatively distinct classes with respect to neurocognitive deficits and neuro-immune biomarkers: results of soft independent modeling of class analogy
IF: 3.584 -
High Mobility Group Protein 1 and Dickkopf-Related Protein 1 in Schizophrenia and Treatment-Resistant Schizophrenia: Associations With Interleukin-6, Symptom Domains, and Neurocognitive Impairments
IF: 7.958 -
Chronic fatigue syndrome and fibromyalgia-like symptoms are an integral component of the phenome of schizophrenia: neuro-immune and opioid system correlates
IF: 2.726 -
RETRACTED ARTICLE: Dickkopf-1 contributes to hepatocellular carcinoma tumorigenesis by activating the Wnt/β-catenin signaling pathway
IF: 5.873 -
Inhibitors of osteoblastogenesis in early human milk and maternal serum: evidence for protective properties of mother’s milk on bone
IF: 1.493Journal： Journal of Maternal-Fetal & Neonatal Medicine (2018)PMID：30122112Reactivity：HumanSample Type：Serum,Milk
实验操作视频
