Human EPO(Erythropoietin) ELISA Kit
中文名称：人促红细胞生成素(EPO)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人EPO抗体包被于酶标板上，实验时样品（或标准品）中的人EPO会与包被抗体结合。后依次加入生物素化的抗人EPO抗体和辣根过氧化物酶标记的亲和素，抗人EPO抗体与结合在包被抗体上的人EPO结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，EPO浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中EPO的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人EPO，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Novel TMPRSS6 variants and their impact on iron-refractory iron deficiency anaemia in pregnancy: A North Indian genotype phenotype study
IF: 5.100Journal： BRITISH JOURNAL OF HAEMATOLOGY (2024)Reactivity：HumanSample Type：Blood -
Serum erythropoietin in acute ischemic stroke: preliminary findings
IF: 4.600 -
Deficiency of lysophosphatidic acid receptor 3 decreases erythropoietin production in hypoxic mouse kidneys
IF: 3.900 -
Iron incorporation in red blood cells of pediatric sickle cell anemia: a stable isotope pilot investigation
IF: 3.600Journal： EUROPEAN JOURNAL OF CLINICAL NUTRITION (2024)Reactivity：HumanSample Type：Serum -
Assessment of Renal Erythropoietic Status of the Newly Diagnosed Diabetic Patients without Renal Impairment in Benin City, Nigeria
IF: 1.900 -
Effects of Hypoxemia by Acute High-Altitude Exposure on Human Intestinal Flora and Metabolism
IF: 4.500 -
COMPARATIVE EVALUATION AND PROGNOSTIC UTILITY OF NEURONAL INJURY BIOMARKERS IN COVID-19 PATIENTS: A PROSPECTIVE STUDY
IF: 3.533 -
Gestational Diabetes Mellitus in Pregnancy Increased Erythropoietin Level Affecting Differentiation Potency of Haematopoietic Stem Cell of Umbilical Cord Blood
IF: 5.093
实验操作视频
