Human FE(Ferritin) ELISA Kit
中文名称：人铁蛋白(FE)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人FE抗体包被于酶标板上，实验时样品（或标准品）中的人FE会与包被抗体结合。后依次加入生物素化的抗人FE抗体和辣根过氧化物酶标记的亲和素，抗人FE抗体与结合在包被抗体上的人FE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人FE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Relationship Between Iron Deficiency and Severity of Tuberculosis: Influence on T Cell Subsets
IF: 4.600Iron absorption in adults with sickle cell anemia: a stable-isotope approach
IF: 4.100Journal： EUROPEAN JOURNAL OF NUTRITION (2024)Reactivity：HumanSample Type：SerumMenstrual cycle does not impact the hypoxic ventilatory response and acute mountain sickness prediction
IF: 3.800Iron incorporation in red blood cells of pediatric sickle cell anemia: a stable isotope pilot investigation
IF: 3.600Journal： EUROPEAN JOURNAL OF CLINICAL NUTRITION (2024)Reactivity：HumanSample Type：SerumIncreased zinc and albumin but lowered copper in children with transfusion-dependent thalassemia
IF: 3.849Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021)PMID：33453474Reactivity：HumanSample Type：serumDapagliflozin Suppresses Hepcidin And Increases Erythropoiesis
IF: 5.399Journal： JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM (2020)PMID：32044999Reactivity：HumanSample Type：plasmaVitamin D3 supplementation for 8 weeks leads to improved haematological status following the consumption of an iron-fortified breakfast cereal: a double-blind randomised controlled trial in iron-deficient women
IF: 3.319Insulin treatment corrects hepcidin but not YKL-40 levels in persons with type 2 diabetes mellitus matched by body mass index, waist-to-height ratio, C-reactive protein and Creatinine
IF: 2.275
