Human FGF23(Fibroblast Growth Factor 23) ELISA Kit
中文名称：人成纤维细胞生长因子23(FGF23)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人FGF23抗体包被于酶标板上，实验时样品（或标准品）中的人FGF23会与包被抗体结合。后依次加入生物素化的抗人FGF23抗体和辣根过氧化物酶标记的亲和素，抗人FGF23抗体与结合在包被抗体上的人FGF23结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FGF23浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FGF23的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人FGF23，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Predictive value of bone metabolism markers in the progression of diabetic kidney disease: a cross-sectional study
IF: 3.900Journal： Frontiers in Endocrinology (2024)Reactivity：HumanSample Type：serum -
The FGF23–Klotho axis promotes microinflammation in chronic kidney disease
IF: 3.700 -
Novel Biomarkers in Evaluating Cardiac Function in Patients on Hemodialysis—A Pilot Prospective Observational Cohort Study
IF: 3.600 -
Baseline fibroblast growth factor 23 predicts incident heart failure and cardiovascular mortality in patients with chronic kidney disease: A 3-year follow-up study
IF: 2.500Journal： IJC Heart & Vasculature (2024)Reactivity：HumanSample Type：serum -
Relationship of Fibroblast Growth Factor 23 Serum Levels with Disease Characteristics in Systemic Lupus Erythematosus Patients
IF: 5.500 -
Correlation study of FGF23/D-serine in maintenance hemodialysis patients with combined hearing impairment
IF: 3.700 -
The roles of Klotho and FGF-23 in bipolar manic episode
IF: 3.300Journal： European Review for Medical and Pharmacological Sciences (2023)Reactivity：HumanSample Type：Venous blood -
An evaluation of both serum Klotho/FGF-23 and apelin-13 for detection of diabetic nephropathy
IF: 3.200Journal： Hormones-International Journal of Endocrinology and Metabolism (2023)PMID：37458962Reactivity：HumanSample Type：serum -
Association of serum 25-hydroxyvitamin D3, fibroblast growth factor-23, and C1q/tumor necrosis factor-related protein-3 with coronary artery calcification in nondialysis chronic kidney disease patients
IF: 3.000 -
Original research: Fibroblast growth factor 23 in children with or without heart failure: a prospective study
IF: 2.600
实验操作视频
