Human GAL3(Galectin 3) ELISA Kit
中文名称：人半乳凝集素3(GAL3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人GAL3抗体包被于酶标板上，实验时样品（或标准品）中的人GAL3会与包被抗体结合。后依次加入生物素化的抗人GAL3抗体和辣根过氧化物酶标记的亲和素，抗人GAL3抗体与结合在包被抗体上的人GAL3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GAL3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GAL3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人GAL3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Galectin-3 induces pathogenic immunosuppressive macrophages through interaction with TREM2 in lung cancer
IF: 11.400Journal： JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2024)Reactivity：HumanSample Type：human lung cancer tissue,normal lung lavage tissue -
Sex-Related Differences in Heart Failure Development in Patients After First Myocardial Infarction: The Role of Galectin-3
IF: 3.900 -
Evaluation of serum galectin-3 concentration as a potential biomarker in exudative-type age-related macular degeneration
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：serum -
Galectin-3 levels in children with cystic fibrosis
IF: 3.600 -
Association between galectin-3 and hepatosteatosis in a community-based cross-sectional study
IF: 3.300Journal： Therapeutic Advances in Chronic Disease (2024)Reactivity：HumanSample Type：Serum -
Predictive Value of NT-proBNP, FGF21, Galectin-3 and Copeptin in Advanced Heart Failure in Patients with Preserved and Mildly Reduced Ejection Fraction and Type 2 Diabetes Mellitus
IF: 2.400 -
Multi-element analysis of metals in human pathological and unchanged thyroid glands – pilot study
IF: 1.900 -
FK506-Binding Protein like (FKBPL) Has an Important Role in Heart Failure with Preserved Ejection Fraction Pathogenesis with Potential Diagnostic Utility
IF: 5.500 -
Elevated Serum Galectin-1 and Galectin-3 Levels in Children With Specific Learning Disorder: A Case Control Study
IF: 2.700 -
The effect of heart failure on gut microbial richness and diversity
IF: 1.800
实验操作视频
