Human GFAP(Glial Fibrillary Acidic Protein) ELISA Kit
中文名称：人神经胶质纤维酸性蛋白(GFAP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人GFAP抗体包被于酶标板上，实验时样品（或标准品）中的人GFAP会与包被抗体结合。后依次加入生物素化的抗人GFAP抗体和辣根过氧化物酶标记的亲和素，抗人GFAP抗体与结合在包被抗体上的人GFAP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GFAP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GFAP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人GFAP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Circulating small extracellular vesicles in Alzheimer’s disease: a case–control study of neuro-inflammation and synaptic dysfunction
IF: 7.000Journal： BMC Medicine (2024)Reactivity：HumanSample Type：plasma-derived small extracellular vesicles (PsEVs) -
In vivo evidence of increased vascular endothelial growth factor in patients with major depressive disorder
IF: 4.900Journal： JOURNAL OF AFFECTIVE DISORDERS (2024)Reactivity：HumanSample Type：plasma -
Levels of biomarkers associated with subconcussive head hits in mixed martial arts fighters
IF: 2.300 -
The effects of hyperbaric oxygen therapy on neuroprotection and recovery after brain resuscitation
IF: 1.700Journal： INTERNATIONAL JOURNAL OF NEUROSCIENCE (2024)Reactivity：HumanSample Type：venous blood -
Cerebrospinal Fluid and Serum Biomarker Insights in Aneurysmal Subarachnoid Haemorrhage: Navigating the Brain–Heart Interrelationship for Improved Patient Outcomes
IF: 4.700 -
Evaluating the Clinical Significance of Diazepam Binding Inhibitor in Alzheimer’s Disease: A Comparison with Inflammatory, Oxidative, and Neurodegenerative Biomarkers
IF: 3.500 -
Inflammation biomarkers IL?6 and IL?10 may improve the diagnostic and prognostic accuracy of currently authorized traumatic brain injury tools
IF: 2.700Journal： Experimental and Therapeutic Medicine (2023)PMID：37408863Reactivity：HumanSample Type：serum -
Plasma biomarkers of brain injury in COVID-19 patients with neurological symptoms
IF: 4.553Journal： JOURNAL OF THE NEUROLOGICAL SCIENCES (2022)PMID：35752131Reactivity：HumanSample Type：serum -
Examination of Cognitive Function, Neurotrophin Concentrations, and both Brain and Systemic Inflammatory Markers Following a Simulated Game of American Football
IF: 4.415Journal： JOURNAL OF STRENGTH AND CONDITIONING RESEARCH (2022)Reactivity：HumanSample Type：serum -
Association between glial fibrillary acidic protein, glial-derived neurotrophic factor, and fatty acid-binding protein-2 at birth in the incidence of necrotizing enterocolitis in preterm infants
IF: 3.569Journal： Frontiers in Pediatrics (2022)Reactivity：HumanSample Type：blood
实验操作视频
