Human GHRL(Ghrelin) ELISA Kit
中文名称：人饥饿素(GHRL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用人GHRL抗原包被于酶标板上，实验时样品或标准品中的人GHRL与包被的人GHRL竞争生物素标记的抗人GHRL单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，人GHRL浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中人GHRL的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人GHRL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Does kisspeptin act as a neuropeptide or as an adipokine in obese people?
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Leu72Met polymorphism of GHRL gene increase the risk factor of obesity in a Javanese ethnic group from Indonesia
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Serum nesfatin-1, ghrelin, and lipid levels in adolescents with first episode drug na?ve unipolar depression
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Effect of Sequence of Fruit Intake in a Meal on Satiety
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Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis
IF: 4.742 -
The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia
IF: 2.528
实验操作视频
