Human Hepc(Hepcidin)ELISA Kit
中文名称：人铁调素(Hepc)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.32 ng/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中Hepc浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人Hepc抗体包被于酶标板上，实验时样品（或标准品）中的人Hepc会与包被抗体结合。后依次加入生物素化的抗人Hepc抗体和辣根过氧化物酶标记的亲和素，抗人Hepc抗体与结合在包被抗体上的人Hepc结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Hepc浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Hepc的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.32 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人Hepc，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Novel TMPRSS6 variants and their impact on iron-refractory iron deficiency anaemia in pregnancy: A North Indian genotype phenotype study
IF: 5.100Journal： BRITISH JOURNAL OF HAEMATOLOGY (2024)Reactivity：HumanSample Type：Blood -
Relationship Between Iron Deficiency and Severity of Tuberculosis: Influence on T Cell Subsets
IF: 4.600 -
Could Hepcidin Be a New Biomarker in Patients with Idiopathic Pulmonary Fibrosis (IPF)?
IF: 3.000Journal： Journal of Clinical Medicine (2024)Reactivity：HumanSample Type：serum -
Erythroferrone and hepcidin levels in children with iron deficiency anemia
IF: 2.000 -
Higher Incidence of Anemia and Clinical Utility of Indices of Iron Homeostasis in Newly Diagnosed Breast Cancer Patients: A Cross-Sectional Study from a Regional Oncology Centre of South India
IF: 1.500Journal： Indian Journal of Clinical Biochemistry (2024)Reactivity：HumanSample Type：serum -
Ochratoxin A Induces Renal Cell Ferroptosis by Disrupting Iron Homeostasis and Increasing ROS
IF: 6.100 -
HBB Gene Mutations and Their Pathological Impacts on HbE/β-Thalassaemia in Kuala Terengganu, Malaysia
IF: 3.600 -
The prebiotic effects of soluble dietary fiber mixture on renal anemia and the gut microbiota in end-stage renal disease patients on maintenance hemodialysis: a prospective, randomized, placebo-controlled study
IF: 8.440 -
Hepcidin expression levels involve efficacy of pegylated interferon-α treatment in hepatitis B-infected liver
IF: 5.714 -
Effect of ultramarathon running on iron metabolism
IF: 3.396Journal： Journal of the Chinese Medical Association (2022)Reactivity：HumanSample Type：Plasma
实验操作视频
