Human Hepc(Hepcidin)ELISA Kit
中文名称：人铁调素(Hepc)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.32 ng/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中Hepc浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人Hepc抗体包被于酶标板上，实验时样品（或标准品）中的人Hepc会与包被抗体结合。后依次加入生物素化的抗人Hepc抗体和辣根过氧化物酶标记的亲和素，抗人Hepc抗体与结合在包被抗体上的人Hepc结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Hepc浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Hepc的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.32 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人Hepc，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Differential effects of high-altitude exposure on markers of oxidative stress, antioxidant capacity, and iron profiles
IF: 3.210Journal： AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY (2022)Reactivity：HumanSample Type：Plasma -
Does the TMPRSS6 C?>?T Polymorphism Modify the Endurance Training Effects on Hematological Parameters?
IF: 3.738 -
Reduced hepcidin expression enhances iron overload in patients with HbE/β?thalassemia: Α comparative cross?sectional study
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34675995Reactivity：HumanSample Type：serum -
Serum hepcidin and interleukin-6 in systemic lupus erythematosus patients: crucial factors for correction of anemia
Journal： Egyptian Rheumatology and Rehabilitation (2020)Reactivity：HumanSample Type：serum -
Increased hepcidin in hemorrhagic plaques correlates with iron-stimulated IL-6/STAT3 pathway activation in macrophages
IF: 2.705 -
Insulin treatment corrects hepcidin but not YKL-40 levels in persons with type 2 diabetes mellitus matched by body mass index, waist-to-height ratio, C-reactive protein and Creatinine
IF: 2.275 -
Applied value of monitoring serum hepcidin in differential diagnosis of infection versus tumor fevers
IF: 0.964Journal： Journal of Huazhong University of Science and Technology-Medical Sciences (2017)PMID：28397053Reactivity：HumanSample Type：Plasma -
An unusual case of iron deficiency anemia is associated with extremely low level of transferrin receptor
IF: 1.891Journal： International Journal of Clinical and Experimental Pathology (2015)DOI：PMID:26339443PMID：26339443Reactivity：HumanSample Type：serum
实验操作视频
