Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
中文名称：人低氧诱导因子1α(HIF-1α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HIF-1α抗体包被于酶标板上，实验时样品（或标准品）中的人HIF-1α会与包被抗体结合。后依次加入生物素化的抗人HIF-1α抗体和辣根过氧化物酶标记的亲和素，抗人HIF-1α抗体与结合在包被抗体上的人HIF-1α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HIF-1α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HIF-1α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HIF-1α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Lipopolysaccharide, VE-cadherin, HMGB1, and HIF-1α levels are elevated in the systemic circulation in chronic migraine patients with medication overuse headache: evidence of leaky gut and inflammation
IF: 7.400 -
Microbiota alterations are related to migraine food triggers and inflammatory markers in chronic migraine patients with medication overuse headache
IF: 7.300Journal： JOURNAL OF HEADACHE AND PAIN (2024)Reactivity：HumanSample Type：Serum -
Association of serum HIF-1α levels with coronary artery calcification in patients of non-dialysis chronic kidney disease
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：Serum -
A Novel Pentapeptide From Tilapia Skin Gelatin Gastrointestinal Digests Which Inhibits Scar Formation Through Anti-Inflammatory and Antiangiogenesis
IF: 3.500 -
The impact of inflammatory and oxidative stress biomarkers on the sympathetic nervous system in severe coronary atherosclerosis
IF: 2.800Journal： Frontiers in Cardiovascular Medicine (2024)Reactivity：HumanSample Type：Peripheral venous blood sample -
Physiological, biochemical and genome-wide expression patterns during graded normobaric hypoxia in healthy individuals
IF: 2.500Journal： PHYSIOLOGICAL GENOMICS (2024)Reactivity：HumanSample Type：serum -
The impact of hsa-miR-1972 on the expression of von Willebrand factor in breast cancer progression regulation
IF: 2.300 -
HIF1α elevations at tissue and serum levels and their association with metabolic disorders in obese children
IF: 5.800Journal： JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM (2023)PMID：38051959Reactivity：HumanSample Type：serum -
Hyperbaric Oxygen Therapy Reduces Oxidative Stress and Inflammation, and Increases Growth Factors Favouring the Healing Process of Diabetic Wounds
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：37108205Reactivity：HumanSample Type：plasma -
Mitochondrial metabolism related markers GDF-15, FGF-21, and HIF-1α are elevated in pediatric migraine attacks
IF: 5.000
实验操作视频
