Human HS(Heparan Sulfate) ELISA Kit
中文名称：人硫酸乙酰肝素(HS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HS抗体包被于酶标板上，实验时样品（或标准品）中的人HS会与包被抗体结合。后依次加入生物素化的抗人HS抗体和辣根过氧化物酶标记的亲和素，抗人HS抗体与结合在包被抗体上的人HS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells
IF: 4.600Journal： Frontiers in Cell and Developmental Biology (2024)Reactivity：HumanSample Type：plasma -
Syndecan-1 Levels and Early Positive Fluid Balance Are Associated with Disease Severity in Acute Pancreatitis
IF: 1.700 -
Functional involvement of endothelial lipase in hepatitis B virus infection
IF: 5.100 -
Effect of intravenous fluid volume on biomarkers of endothelial glycocalyx shedding and inflammation during initial resuscitation of sepsis
IF: 3.500Journal： Intensive Care Medicine Experimental (2023)PMID：37062769Reactivity：HumanSample Type：plasma -
Multimodal measurement of glycocalyx degradation during coronary artery bypass grafting
IF: 5.058Journal： Frontiers in Medicine (2022)Reactivity：HumanSample Type：blood -
Doxycycline can reduce glycocalyx shedding by inhibiting matrix metalloproteinases in patients undergoing cardiopulmonary bypass: A randomized controlled trial
IF: 3.750 -
Endothelial glycocalyx during early reperfusion in patients undergoing cardiac surgery
IF: 3.240 -
Endothelial glycocalyx degradation is associated with early organ impairment in polytrauma patients
IF: 2.119 -
Graft glycocalyx degradation in human liver transplantation
IF: 2.776
实验操作视频
