Human HSP-70/HSPA9(Heat Shock 70 kDa Protein 9) ELISA Kit
中文名称：人热休克蛋白70(HSP-70/HSPA9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HSP-70/HSPA9抗体包被于酶标板上，实验时样品（或标准品）中的人HSP-70/HSPA9会与包被抗体结合。后依次加入生物素化的抗人HSP-70/HSPA9抗体和辣根过氧化物酶标记的亲和素，抗人HSP-70/HSPA9抗体与结合在包被抗体上的人HSP-70/HSPA9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HSP-70/HSPA9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HSP-70/HSPA9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人HSP-70/HSPA9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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实验操作视频
