Human IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit
中文名称：人吲哚胺2,3-双加氧酶(IDO)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IDO抗体包被于酶标板上，实验时样品（或标准品）中的人IDO会与包被抗体结合。后依次加入生物素化的抗人IDO抗体和辣根过氧化物酶标记的亲和素，抗人IDO抗体与结合在包被抗体上的人IDO结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IDO浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IDO的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IDO，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Overexpression of serum HMGB1 and IDO in esophageal squamous cell carcinoma patients: potential clinical auxiliary diagnostic markers and immunotherapeutic targets
IF: 3.500Journal： Frontiers in Oncology (2024)Reactivity：HumanSample Type：serum -
Design, synthesis and evaluation the bioactivities of novel 1,3-dimethyl-6-amino-1H-indazole derivatives as anticancer agents
IF: 3.500 -
Activities of plasma indoleamine-2, 3-dioxygenase (IDO) enzyme in Nigerian patients with lung diseases: basis for tryptophan supplementation or IDO inhibitor use
IF: 1.300Journal： Egyptian Journal of Bronchology (2023)Reactivity：HumanSample Type：plasma -
Enforced mesenchymal stem cell tissue colonization counteracts immunopathology
IF: 14.404 -
Proteinuria is Associated with Urinary Loss of Cubilin and Vitamin D-Binding Protein in Patients with Preeclampsia
IF: 3.998 -
1-MT inhibits the invasion of CBP-resistant ovarian cancer cells via down-regulating IDO expression and re-activating immune cells function
IF: 1.771 -
Design, synthesis and biological evaluation of a novel library of antimitotic C2-aroyl/arylimino tryptamine derivatives that are also potent inhibitors of indoleamine-2, 3-dioxygenase (IDO)
IF: 3.466
实验操作视频
