Human IFNα(Interferon Alpha ) ELISA Kit
中文名称:人干扰素α(IFNα)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFNα抗体包被于酶标板上,实验时样品(或标准品)中的人IFNα会与包被抗体结合。后依次加入生物素化的抗人IFNα抗体和辣根过氧化物酶标记的亲和素,抗人IFNα抗体与结合在包被抗体上的人IFNα结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFNα浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFNα的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IFNα,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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The Antiviral Effects of Heat-Killed Lactococcus lactis Strain Plasma Against Dengue, Chikungunya, and Zika Viruses in Humans by Upregulating the IFN-α Signaling Pathway
IF: 4.100 -
Serum interferon-alpha predicts in-hospital mortality in patients hospitalised with acute severe lupus
IF: 3.900 -
Nailfold capillaroscopy in Egyptian systemic lupus erythematosus (SLE) patients: correlation with demographic features and serum levels of IL 17A and IFNs I
IF: 0.800Journal: Egyptian Rheumatology and Rehabilitation (2023)Reactivity:HumanSample Type:Serum -
OA11?Serum interferon alpha levels as a predictor of short-term mortality in patients of SLE with high disease activity
IF: 7.046Journal: RHEUMATOLOGY (2022)Reactivity:HumanSample Type:serum -
miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus
IF: 4.631 -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657Journal: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022)PMID:36179036Reactivity:HumanSample Type:fecal -
Differential immune responses in pregnant patients recovered from COVID-19
IF: 18.187Journal: Signal Transduction and Targeted Therapy (2021)PMID:34326311Reactivity:HumanSample Type:blood -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
2-O-Methylhonokiol Suppresses HCV Replication via TRAF6-Mediated NF-kB Activation
IF: 5.924 -
Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells
IF: 2.352Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020)PMID:32048561Reactivity:HumanSample Type:Cell culture supernatant
Q1:人干扰素α(IFNα)酶联免疫吸附测定试剂盒, 检测哪种亚型IFNα? 如果检测的是总的,那标准品是混合物吗?
该指标测得是所有亚型的IFNα,也就是总的IFNα。标准品并不是所有亚型的混合物,而是人源的IFN-alpha 2a (重组蛋白,表达体系:HEK293细胞)。但由于人的15种IFN-α亚型同源性高达80%,所以我们试剂盒中所用到的抗体可识别所有亚型,另外这款试剂盒对不同亚型的IFNα做过验证,均是可以检测到的。