Human IFN-β(Interferon Beta) ELISA Kit
中文名称：人β干扰素(IFN-β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-β抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-β会与包被抗体结合。后依次加入生物素化的抗人IFN-β抗体和辣根过氧化物酶标记的亲和素，抗人IFN-β抗体与结合在包被抗体上的人IFN-β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Reactivation of senescence-associated endogenous retroviruses by ATF3 drives interferon signaling in aging
IF: 17.000 -
DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants
IF: 16.600 -
STING inhibitors sensitize platinum chemotherapy in ovarian cancer by inhibiting the CGAS-STING pathway in cancer-associated fibroblasts (CAFs)
IF: 9.700 -
Manganese inhibits HBV transcription and promotes HBsAg degradation at non-toxic levels
IF: 7.700Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：Human -
Influenza A virus infection activates caspase-8 to enhance innate antiviral immunity by cleaving CYLD and blocking TAK1 and RIG-I deubiquitination
IF: 6.200Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)Reactivity：Human -
Foot-and-Mouth Disease Virus Capsid Protein VP1 Antagonizes Type I Interferon Signaling via Degradation of Histone Deacetylase 5
IF: 6.000 -
Interferon-γ in the tumor microenvironment promotes the expression of B7H4 in colorectal cancer cells, thereby inhibiting cytotoxic T cells
IF: 4.600 -
Loss of ZNF408 attenuates STING-mediated immune surveillance in breast carcinogenesis
IF: 4.600 -
CD47-targeted immunotherapy unleashes antitumour immunity in Epstein-Barr virus-associated gastric cancer
IF: 8.600 -
A novel lncRNA DFRV plays a dual function in influenza A virus infection
IF: 5.200
实验操作视频
