Human IFN-β(Interferon Beta) ELISA Kit
中文名称：人β干扰素(IFN-β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-β抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-β会与包被抗体结合。后依次加入生物素化的抗人IFN-β抗体和辣根过氧化物酶标记的亲和素，抗人IFN-β抗体与结合在包被抗体上的人IFN-β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling
IF: 4.328Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2019)PMID：30826466Reactivity：HumanSample Type：Serum -
Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways.
IF: 2.173 -
Encephalomyocarditis virus 2C protein antagonizes interferon-β signaling pathway through interaction with MDA5
IF: 4.307 -
The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3
IF: 6.429Journal： Frontiers in Immunology (2017)PMID：28717359Reactivity：HumanSample Type：Cell culture supernatant -
Influenza A Virus PA Antagonizes Interferon-β by Interacting with Interferon Regulatory Factor 3
IF: 6.429Journal： Frontiers in Immunology (2017)PMID：28955326Reactivity：HumanSample Type：Cell culture supernatant -
A Novel Function of F-Box Protein FBXO17 in Negative Regulation of Type I IFN Signaling by Recruiting PP2A for IFN Regulatory Factor 3 Deactivation
IF: 4.856Journal： JOURNAL OF IMMUNOLOGY (2017)PMID：27956528Reactivity：HumanSample Type：Cell culture supernatant -
Matrix Metalloproteinase 9 Facilitates Hepatitis B Virus Replication through Binding with Type I Interferon (IFN) Receptor 1 To Repress IFN/JAK/STAT Signaling
IF: 4.663Journal： JOURNAL OF VIROLOGY (2017)PMID：28122987Reactivity：HumanSample Type：Cell culture supernatant -
miR-194 Inhibits Innate Antiviral Immunity by Targeting FGF2 in Influenza H1N1 Virus Infection
IF: 4.076Journal： Frontiers in Microbiology (2017)PMID：29163456Reactivity：Human,MouseSample Type：Cell culture supernatant,Bronchoalveolar Lavage Fluid -
Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways
IF: 2.956Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2017)PMID：29153953Reactivity：HumanSample Type：Cell culture supernatant -
Hepatitis C virus infection decreases the expression of Toll-like receptors 3 and 7 via upregulation of miR-758
IF: 2.282Journal： ARCHIVES OF VIROLOGY (2014)PMID：25008898Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
