Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Extracellular vesicle-packaged lncRNA from cancer-associated fibroblasts promotes immune evasion by downregulating HLA-A in pancreatic cancer
IF: 15.500 -
Sex-specific circulating unconventional neutrophils determine immunological outcome of auto-inflammatory Beh?et’s uveitis
IF: 13.000 -
Single-cell data-driven design of armed oncolytic virus and combination therapy to activate a cooperative innate-adaptive immunity against cancer
IF: 12.100 -
Tumor-associated macrophage enhances PD-L1-mediated immune escape of bladder cancer through PKM2 dimer-STAT3 complex nuclear translocation
IF: 9.100 -
Cannabidiol exerts multitarget immunomodulatory effects on PBMCs from individuals with psoriasis vulgaris
IF: 7.300 -
Ginsenoside Rg3 activates the immune function of CD8+?T cells via circFOXP1-miR-4477a-PD-L1 axis to induce ferroptosis in gallbladder cancer
IF: 6.900 -
Modulation of innate immunity related genes resulting in prophylactic antimicrobial and antiviral properties
IF: 6.100 -
C3+ cancer-associated fibroblasts promote tumor growth and?therapeutic resistance in gastric cancer via activation of the NF-κB signaling pathway
IF: 6.100 -
Anti-Tumor Immunity to Patient-Derived Breast Cancer Cells by Vaccination with Interferon-Alpha-Conditioned Dendritic Cells (IFN-DC)
IF: 5.200 -
Hypomethylation‐associated ELF3 helps nasopharyngeal carcinoma to escape immune surveillance via MUC16-mediated glycolytic metabolic reprogramming
IF: 5.000Journal： AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2024)Reactivity：Human
实验操作视频
