Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
The correlation between TRIM28 expression and immune checkpoints in CRPC
IF: 4.400 -
Single-cell RNA Sequencing Reveals the Diversity of the Immunological Landscape Response to Genital Herpes
IF: 4.300Journal： VIROLOGICA SINICA (2024)Reactivity：HumanSample Type：plasma -
Molecular mechanisms of transmitted endoplasmic reticulum stress mediating immune escape of gastric cancer via PVR overexpression in TAMs
IF: 4.200Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)Reactivity：Human -
Unveiling immune tolerance pathways in preeclampsia placenta: implications for molecular targets and discovery of potential biomarkers
IF: 3.900Journal： Frontiers in Endocrinology (2024)Reactivity：HumanSample Type：placental tissue -
Lifestyle deterioration linked to elevated inflammatory cytokines over a two-month follow-up
IF: 3.800Journal： Scientific Reports (2024)Reactivity：HumanSample Type：venous blood tissue -
An ayurvedic approach to immunomodulation employing a green delivery of phytonutrients: A randomized, double-blind, placebo-controlled study on mild allergic rhinitis
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：HumanSample Type：Serum -
Augmented IFNγ producing ILC1 and IL 17 producing ILC3 in pemphigus vulgaris: Plausible therapeutic target
IF: 3.700Journal： CELLULAR IMMUNOLOGY (2024)Reactivity：HumanSample Type：serum -
Human amniotic membrane scaffold enhances adipose mesenchymal stromal cell mitochondrial bioenergetics promoting their regenerative capacities
IF: 3.500 -
Curzerenone inactivates the nuclear factor-kappa B signaling to suppress malignancy and immune evasion in cervical cancer by targeting CSNK2B
IF: 3.400 -
Distorted frequency and functionality of natural killer cells in pemphigus vulgaris: A potential therapeutic target
IF: 3.300
实验操作视频
