Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Augmented IFNγ producing ILC1 and IL 17 producing ILC3 in pemphigus vulgaris: Plausible therapeutic target
IF: 3.700Journal： CELLULAR IMMUNOLOGY (2024)Reactivity：HumanSample Type：serum -
Human amniotic membrane scaffold enhances adipose mesenchymal stromal cell mitochondrial bioenergetics promoting their regenerative capacities
IF: 3.500 -
Curzerenone inactivates the nuclear factor-kappa B signaling to suppress malignancy and immune evasion in cervical cancer by targeting CSNK2B
IF: 3.400 -
Distorted frequency and functionality of natural killer cells in pemphigus vulgaris: A potential therapeutic target
IF: 3.300 -
Knockdown of PELI1 promotes Th2 and Treg cell differentiation in juvenile idiopathic arthritis
IF: 3.300Journal： EXPERIMENTAL CELL RESEARCH (2024)Reactivity：HumanSample Type：serum -
Tilianin enhances the antitumor effect of sufentanil on non-small cell lung cancer
IF: 3.200Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024)Reactivity：Human -
15-Deoxy-Δ-12,14-Prostaglandin J2 Represses Immune Escape of Lung Adenocarcinoma by Polarizing Macrophages Through Epidermal Growth Factor Receptor/Ras/Raf Pathway
IF: 3.200 -
B7-H3 promotes nasopharyngeal carcinoma progression by regulating CD8+ T cell exhaustion
IF: 3.100 -
The role of immune-inflammatory markers in children with complicated and uncomplicated malaria in Enugu, Nigeria
IF: 2.900 -
Spatial and Temporal Distribution Characteristics and Cytotoxicity of Atmospheric PM2.5 in the Main Urban Area of Lanzhou City
IF: 2.700Journal： BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY (2024)Reactivity：Human
实验操作视频
