Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Serum mir-142-3p release in children with viral encephalitis and its relationship with nerve injury and inflammatory response
IF: 2.300Journal： JOURNAL OF NEUROVIROLOGY (2024)Reactivity：HumanSample Type：serum -
Pereskia bleo augments NK cell cytotoxicity against triple-negative breast cancer cells (MDA-MB-231)
IF: 2.300 -
Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3
IF: 2.000 -
circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity
IF: 1.500 -
Discovery and biological evaluation of a potent small molecule CRM1 inhibitor for its selective ablation of extranodal NK/T cell lymphoma
IF: 7.700 -
The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ
IF: 4.345Journal： OncoTargets and Therapy (2022)PMID：30425515Reactivity：HumanSample Type：Cell culture supernatant -
Differential immune responses in pregnant patients recovered from COVID-19
IF: 18.187Journal： Signal Transduction and Targeted Therapy (2021)PMID：34326311Reactivity：HumanSample Type：blood -
Abrogation of HnRNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8+ T cell-mediated ferroptosis in castration-resistant prostate cancer
IF: 11.614 -
Rational design of a prodrug to inhibit self-inflammation for cancer treatment
IF: 7.790Journal： Nanoscale (2021)PMID：33710220Reactivity：MouseSample Type：Serum,Cell culture supernatant(dendritic cells,4T1 cells,RAW 264.7) -
Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis
IF: 5.742
实验操作视频
