Human IFN-γ(Interferon Gamma) ELISA Kit

中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒

货号：E-EL-H0108
文献（144）
说明书

说明书

别称：

IFNG、IFG、IFI

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Human
检测范围： 15.63-1000 pg/mL
灵敏度： 9.38 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Human
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.38 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Interferon-γ in the tumor microenvironment promotes the expression of B7H4 in colorectal cancer cells, thereby inhibiting cytotoxic T cells

IF: 4.6

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-56681-3

Sample Type：Peripheral blood mononuclear cells (PBMCs),human umbilical vein endothelial cells (HUVECs)
Loss of ZNF408 attenuates STING-mediated immune surveillance in breast carcinogenesis

IF: 4.6

Journal： iScience (2024)

DOI：10.1016/j.isci.2024.110276

Reactivity：Human
YTHDF3 phase separation regulates HSPA13-dependent clear cell renal cell carcinoma development and immune evasion

IF: 4.5

Journal： CANCER SCIENCE (2024)

DOI：10.1111/cas.16228

Reactivity：Human
The correlation between TRIM28 expression and immune checkpoints in CRPC

IF: 4.4

Journal： FASEB JOURNAL (2024)

DOI：10.1096/fj.202400061RR

Reactivity：Human
Single-cell RNA Sequencing Reveals the Diversity of the Immunological Landscape Response to Genital Herpes

IF: 4.3

Journal： VIROLOGICA SINICA (2024)

DOI：10.1016/j.virs.2024.10.003

Reactivity：Human

Sample Type：plasma
Molecular mechanisms of transmitted endoplasmic reticulum stress mediating immune escape of gastric cancer via PVR overexpression in TAMs

IF: 4.2

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)

DOI：10.1016/j.bbadis.2024.167560

Reactivity：Human
Unveiling immune tolerance pathways in preeclampsia placenta: implications for molecular targets and discovery of potential biomarkers

IF: 3.9

Journal： Frontiers in Endocrinology (2024)

DOI：10.3389/fendo.2024.1385154

Reactivity：Human

Sample Type：placental tissue
Lifestyle deterioration linked to elevated inflammatory cytokines over a two-month follow-up

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-69967-3

Reactivity：Human

Sample Type：venous blood tissue
An ayurvedic approach to immunomodulation employing a green delivery of phytonutrients: A randomized, double-blind, placebo-controlled study on mild allergic rhinitis

IF: 3.8

Journal： Journal of Functional Foods (2024)

DOI：10.1016/j.jff.2024.106619

Reactivity：Human

Sample Type：Serum
Augmented IFNγ producing ILC1 and IL 17 producing ILC3 in pemphigus vulgaris: Plausible therapeutic target

IF: 3.7

Journal： CELLULAR IMMUNOLOGY (2024)

DOI：10.1016/j.cellimm.2024.104910

Reactivity：Human

Sample Type：serum

Species

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Canine

Human

Mouse

Porcine

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