Human IFN-γ(Interferon Gamma) ELISA Kit
中文名称：人γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的人IFN-γ会与包被抗体结合。后依次加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗人IFN-γ抗体与结合在包被抗体上的人IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Immunosuppression Induced by Glutamine Deprivation Occurs via Activating PD-L1 Transcription in Bladder Cancer
IF: 5.246 -
Glucose metabolism involved in PD-L1-mediated immune escape in the malignant kidney tumour microenvironment
IF: 5.241Journal： Cell Death Discovery (2021)PMID：33462221Reactivity：HumanSample Type：Cell culture supernatant -
Critically Ill Coronavirus Disease 2019 Patients Exhibit Hyperactive Cytokine Responses Associated With Effector Exhausted Senescent T Cells in Acute Infection
IF: 5.226Journal： JOURNAL OF INFECTIOUS DISEASES (2021)Reactivity：HumanSample Type：serum -
Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
IF: 4.932 -
Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis
IF: 4.575Journal： INFLAMMATION RESEARCH (2021)PMID：33852061Reactivity：MouseSample Type：Serum,Bronchoalveolar Lavage Fluid -
Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion
IF: 4.380 -
Combination of oncolytic adenovirus ZD55 harboring TRAIL-IETD-MnSOD and cytokine-induced killer cells against lung cancer
IF: 3.932Journal： Annals of Translational Medicine (2021)PMID：34790733Reactivity：Human,MouseSample Type：serum -
Genetic Polymorphisms of IFNG, IFNGR1, and Androgen Receptor and Chronic Prostatitis/Chronic Pelvic Pain Syndrome in a Chinese Han Population
IF: 3.434 -
Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens
IF: 3.317Journal： BMC Pulmonary Medicine (2021)PMID：34930201Reactivity：HumanSample Type：serum, nasal lavage -
Evaluation of IL-10, IFN-γ, and thiol–disulfide homeostasis in patients with drug-resistant epilepsy
IF: 3.307
实验操作视频
